We conducted a retrospective study in the Department of Neonatology and Neonatal Intensive Care Unit in CHU Hassan II Fez including newborns hospitalized in 2012 for urinary tract infection. The aim was to determine the presenting symptoms, the results of the urine culture and management modalities. We included during this period 53 cases that met our criteria. In our study, we found a male predominance. Warning signs were dominated by jaundice (54%). Urinary signs were present in 12 % of cases. The association of leucocyturia and a significant bacteriuria was present in 66 % of our patients. E. coli was the most common organism. Susceptibility to aminopenicillin, to 3rd generation cephalosporins and gentamicin were 14%, 73% and 86% respectively. CRP was greater than 20 mg/l in 32% of cases. Blood culture was positive in 6 % of cases. 43% of patients were treated and followed in ambulatory and the other half was hospitalized. Factors that indicated hospitalization were hemodynamic instability, acute dehydration, kidney failure, presence of sepsis and failure after initial treatment. Ultrasound revealed pyelocalyceal dilation in 9.4% of cases ureterohydronephrosis in 7.5% of cases and nephrocalcinosis in 4% of cases. Retrograde Urethro Cystography confirmed two cases of vesicoureteral reflux. And we also found a case of unilateral renal dysplasia. The first-line empiric antibiotic therapy was 3rd generation cephalosporin – Gentamicin association which was prescribed in 94.3 % of our patients. The minimum duration of treatment was 10 days. The clinical and biological evolution was favorable in most patients. 3 cases of recurrence infection was diagnosed and treated.Urinary tract infection present a high frequency in neonates. The symptoms are dominated by jaundice. The E. coli is the most identified organism with significant resistance to aminopenicillin, while maintaining a good sensitivity to 3rd generation cephalosporins